Sex-dependent differences in the secretome of human endothelial cells
نویسندگان
چکیده
Abstract Background Cellular sex has rarely been considered as a biological variable in preclinical research, even when the pathogenesis of diseases with predictable differences is studied. In this perspective, proteomics, and “omics” approaches general, can provide powerful tools to obtain comprehensive cellular maps, thus favoring discovery still unknown sex-biased physio-pathological mechanisms. Methods We performed proteomic Gene Ontology (GO) analyses secretome from human serum-deprived male female endothelial cells (ECs) followed by ELISA validation. Apoptosis was detected FACS Western blot techniques efferocytosis through ability macrophage cell line RAW 264.7 engulf apoptotic ECs. PTX3 mRNA levels were measured RT-qPCR. Results Proteomic GO starved ECs demonstrated significant enrichment proteins related responses stress regulation apoptosis Accordingly, higher percentage underwent response serum deprivation comparison Among secreted proteins, we reliably found EC secretome. The silencing suggested that dependent on its expression properly carry out efferocytotic process. At variance, seemed be independent expression. Conclusions Our results serum-starved possess different secretory phenotypes might take part stress. identified crucial player male-specific an trigger. This novel sex-related role for mainly PTX3, may open way sex-specific mechanisms pharmacological targets prevention treatment dysfunction at onset atherosclerosis cardiovascular disease.
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ژورنال
عنوان ژورنال: Biology of Sex Differences
سال: 2021
ISSN: ['2042-6410']
DOI: https://doi.org/10.1186/s13293-020-00350-3